INFORMED-SPORT tests products/ingredients for a range of substances that appear on the WADA Prohibited List, as well as lists from organisations such as the NFL, NCAA, and MLB. Substances may be included in the testing specification for a number of reasons, including:
The range of substances included is reviewed regularly against current knowledge and intelligence, and is updated as necessary. It includes anabolic agents, narcotics, stimulants, beta-2-agonists, diuretics, etc.
The INFORMED-SPORT programme uses HFL Sport Science to carry out its supplement analysis. HFL has been testing supplements/ingredients for banned substances since 2002 and tests over 5000 samples each year. The standard supplement screening test covers 146 substances that are prohibited in sport. Additional substances can also be tested for on request.
Click here for examples of substances that are prohibited in sport that could be found as contaminants in supplements.
Methylhexaneamine: In 2010, a number of nutritional supplement products have been implicated in disciplinary hearings associated with doping violations for the compound methylhexaneamine. As part of the INFORMED-SPORT programme, all registered products undergo a rigorous testing regime, which includes analysis for methylhexaneamine, along with a wide range of other prohibited compounds. Athletes, coaches and manufacturers alike, can have the confidence that screening procedures have not identified methylhexaneamine within ANY products registered on the INFORMED-SPORT programme.
Citrus aurantium: Citrus aurantium (bitter orange) is a plant derived ingredient that is used in a number of foods, including some nutritional supplements. Bitter orange naturally contains the stimulant synephrine as well as small amounts of octopamine. Currently, octopamine appears on the WADA list of stimulants that are prohibited for athletes to use in competition. Synephrine, however, is part of the WADA monitoring programme. In addition to identifying the presence of octopamine within products containing Citrus aurantium, screening tests performed by HFL Sport Science have indicated the presence of octopamine within a wide range of natural 'orange based' food products including freshly squeezed orange juice, marmalades, orange squash, etc. These products should not pose a doping risk to athletes. HFL is working closely with sports authorities and advisors to ensure that supplements and foods containing bitter orange do not pose an undue risk to an athlete when consumed as directed and as part of a normal diet.1
IGF-1: HFL Sport Science does not routinely test supplements or supplement ingredients for the presence of IGF-1, as peer-reviewed scientific publications indicate that IGF-1 is not absorbed via the gut following oral ingestion.2,3
Androstenedione: The test sensitivity for anabolic steroids in the standard HFL supplement screen is 10 ng/g (10 ppb). Androstenedione is known to be naturally present in milk and milk-derived products. The concentrations of androstenedione found in milk are variable, but are typically in the low ng/ml (low ppb) region. One reference cites values of around 3.5 ng/ml. For this reason, the presence of androstenedione in milk or milk-derived supplement products and/or supplement ingredients analysed using the standard HFL supplement screen is not reported unless the concentration exceeds 50 ng/g (50 ppb).4
1Thevis, M., Koch, A., Sigmund, G., Thomas, A., Schänzer, W. Analysis of octopamine in human doping control samples. Biomed. Chromatogr. 2011
2Kuipers, H., et al. Effects of Oral Bovine Colostrum Supplementation on Serum Insulin-like Growth Factor-1 Levels. Nutrition 2002, 18:566-567.
3Mero, A., et al. IGF-1, IgA and IgG responses to bovine colostrum supplementation during training. J. Appl. Physiol 2002, 93:732-9.
4Gaiani, R., et al. Androstenedione and Testosterone Concentrations in Plasma and Milk of the Cow throughout Pregnancy. J. Reprod. Fert. 1984, 70: 55-59.